Complete nucleotide sequence of capsicum chlorosis virus isolated from Phalaenopsis orchid and the prediction of the unexplored genetic information of tospoviruses

作者: You-Xiu Zheng , Ching-Chung Chen , Fuh-Jyh Jan

DOI: 10.1007/S00705-010-0874-Y

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摘要: Phalaenopsis orchids are popular ornamentals all over the world. A tospovirus, capsicum chlorosis virus (CaCV-Ph) had been identified as cause of chlorotic ringspots on leaves in Taiwan. The tripartite genome CaCV-Ph was found to contain 3608, 4848 and 8916 nt S, M L RNAs, respectively. Phylogenetic analysis nucleocapsid (N) protein confirmed that is a member watermelon silver mottle (WSMoV) serogroup genus Tospovirus. Based relations among nonstructural (NSs), glycoprotein (GnGc), thrips genera, host geographical distribution, tospoviruses could be classified into two major types: WSMoV-Thrips-Asian Tomato spotted wilt (TSWV)-Frankliniella-EuroAmerican. proline (P459) tospoviral Gn proteins indispensable for transmission, but RGD motif, which maintained by only six tospoviruses, may not required transmission. An RdRp catalytic domain conserved region recognize typically sequences 5’ 3’ terminal regions (5’ AGAGCAAU 3’).

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