Label-free in vivo cellular-level detection and imaging of apoptosis
作者： Andrew J. Bower , Marina Marjanovic , Youbo Zhao , Joanne Li , Eric J. Chaney
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摘要: Cell death plays a critical role in health and homeostasis as well as in the pathogenesis and treatment of a broad spectrum of diseases and can be broadly divided into two main categories: apoptosis, or programmed cell death, and necrosis, or acute cell death. While these processes have been characterized extensively in vitro, label‐free detection of apoptosis and necrosis at the cellular level in vivo has yet to be shown. In this study, for the first time, fluorescence lifetime imaging microscopy (FLIM) of intracellular reduced …
sci-hub.se 参考文章(40)
Roxana M. Vlad, Michael C. Kolios, Gregory J. Czarnota, Ultrasound imaging of apoptosis: spectroscopic detection of DNA-damage effects at high and low frequencies. Methods of Molecular Biology. ,vol. 682, pp. 165- 187 ,(2011) , 10.1007/978-1-60327-409-8_13
G Koopman, CP Reutelingsperger, GA Kuijten, RM Keehnen, ST Pals, MH van Oers, Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood. ,vol. 84, pp. 1415- 1420 ,(1994) , 10.1182/BLOOD.V84.5.1415.1415
Jagat Narula, Elmo R. Acio, Navneet Narula, Louis E. Samuels, Billy Fyfe, Diana Wood, Jane M. Fitzpatrick, P.N. Raghunath, John E. Tomaszewski, Christine Kelly, Neil Steinmetz, Allan Green, John F. Tait, Jeffrey Leppo, Francis G. Blankenberg, Diwakar Jain, H. William Strauss, Annexin-V imaging for noninvasive detection of cardiac allograft rejection Nature Medicine. ,vol. 7, pp. 1347- 1352 ,(2001) , 10.1038/NM1201-1347
M. Okada, N. I. Smith, A. F. Palonpon, H. Endo, S. Kawata, M. Sodeoka, K. Fujita, Label-free Raman observation of cytochrome c dynamics during apoptosis Proceedings of the National Academy of Sciences of the United States of America. ,vol. 109, pp. 28- 32 ,(2012) , 10.1073/PNAS.1107524108
Weihai Ying, NAD+/NADH and NADP+/NADPH in Cellular Functions and Cell Death: Regulation and Biological Consequences Antioxidants & Redox Signaling. ,vol. 10, pp. 179- 206 ,(2008) , 10.1089/ARS.2007.1672
F. G. Blankenberg, P. D. Katsikis, J. F. Tait, R. E. Davis, L. Naumovski, K. Ohtsuki, S. Kopiwoda, M. J. Abrams, M. Darkes, R. C. Robbins, H. T. Maecker, H. W. Strauss, In vivo detection and imaging of phosphatidylserine expression during programmed cell death Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 6349- 6354 ,(1998) , 10.1073/PNAS.95.11.6349
John F. R. Kerr, Clay M. Winterford, Brian V. Harmon, Apoptosis. Its significance in cancer and cancer Therapy Cancer. ,vol. 73, pp. 2013- 2026 ,(1994) , 10.1002/1097-0142(19940415)73:8<2013::AID-CNCR2820730802>3.0.CO;2-J
Heather G. Keen, Bronwen A. Dekker, Lynn Disley, David Hastings, Steve Lyons, Andrew J. Reader, Penny Ottewell, Alastair Watson, Jamal Zweit, Imaging apoptosis in vivo using 124I-annexin V and PET. Nuclear Medicine and Biology. ,vol. 32, pp. 395- 402 ,(2005) , 10.1016/J.NUCMEDBIO.2004.12.008
C. Thompson, Apoptosis in the pathogenesis and treatment of disease Science. ,vol. 267, pp. 1456- 1462 ,(1995) , 10.1126/SCIENCE.7878464
Jonathan M. Levitt, Amy Baldwin, Antonios Papadakis, Sameer Puri, Joanna Xylas, Karl Münger, Irene Georgakoudi, Intrinsic fluorescence and redox changes associated with apoptosis of primary human epithelial cells. Journal of Biomedical Optics. ,vol. 11, pp. 064012- 064012 ,(2006) , 10.1117/1.2401149