Specificity of microRNA target selection in translational repression
作者： John G Doench , Phillip A Sharp
DOI: 10.1101/GAD.1184404
关键词:
摘要: MicroRNAs (miRNAs) are a class of noncoding RNAs found in organisms as evolutionarily distant plants and mammals, yet most the mRNAs they regulate unknown. Here we show that ability an miRNA to translationally repress target mRNA is largely dictated by free energy binding first eight nucleotides 5' region miRNA. However, G:U wobble base-pairing this interferes with activity beyond predicted on basis thermodynamic stability. Furthermore, can be simultaneously repressed more than one species. The level repression achieved dependent both amount available complexes. Thus, miRNA:mRNA interactions must viewed context other potential cellular conditions.
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