作者: AHMM Reza , SF Rakhi , MS Hossen , K Takahashi , Z Hossain
DOI: 10.1007/S10695-012-9740-Z
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摘要: Incorporation of polyunsaturated fatty acids (PUFAs) as biofunctional compounds with feed is an effective way for gonadal maturation without any hazardous effects on animal health, and thus it possible to save the vulnerable species from danger extinction. In present study sperm quality, level Ca2+ concentration in serum, histological structure liver developmental stages ovary endangered fish species, Nandus nandus were investigated confirmation positive PUFAs reproduction maturation. Fishes collected Brahmaputra River, Mymensingh, Bangladesh. Treated group was fed 1 % squid extracted phospholipid supplemented diet that mixed silver carp muscle where controlled same except phospholipid. For histology gonads, samples dehydrated, cleaned infiltrated, embedded paraffin wax sectioned. After that, stained hematoxylin eosin. The photomicrographs taken by using light microscope. comparison control group, treated exhibited higher which resulted spontaneous spawning. female demonstrated advanced during different months. During spawning season, lipid granules normal morphological alteration observed case liver, whereas less more group. Serum found significantly (P < 0.01) contrast breeding season indicator augment estrogen secretion ovarian Better early oocytes, hepatocytes performances PUFA-treated a result efficiency enhancing experiment suggests supplementation dietary improve fish.