Endogenous GD3 ganglioside induces apoptosis in U‐1242 MG glioma cells

摘要: GD3 ganglioside induces apoptosis in several cell types, but the molecular events through which this occurs are largely unknown. We investigated apoptotic effects of expression using U-1242 MG glioblastoma cells, as these cells synthesize almost exclusively GM3 and GM2 not GD3. To express under control TetOn system with minimum leakage, we modified by constructing a single tri-cistronic retrovirus vector containing three genes separated two internal ribosome entry sites: (a) transcriptional silencer, tTS; (b) mutant reverse activator, rtTA2(S)-M2 (provided H. Bujard, Heidelberg, Germany); (c) enhanced green fluorescent protein (EGFP), an indicator gene expression. Using flow cytometry, selected glioma (U1242MG-GD3 clone) that high levels response to doxycycline. Expression was associated verified annexin-V binding, TdT-mediated dUTPnick end-labelling assay (TUNEL), EGFP degradation. GD3-induced occurred via caspase-8 activation, caused cleavage inhibition activation zlETD-fmk minimized apoptosis.