Development of a tightly-regulated tetracycline-dependent transcriptional activator and repressor co-expression system for the strong induction of transgene expression

摘要: The teteracycline (Tc)-dependent and -inducible transcriptional activator (rtTA) system has been used to express regulated transgene expression in vitro vivo. However, previous reports have demonstrated that, even the absence of Tc, rtTA binds weakly tetracycline response element (TRE), leading a low level background activity. In order reduce leaky gene induced by rtTA, we previously established tightly (A-IRES-R system) that makes use both (A) Tc-dependent repressor (TetR-Kruppel-associated box; KRAB) (R). addition, others described transactivator rtTA2-M2 (M2) displays higher sensitivity Dox than rtTA. this study, further develop A-IRES-R system, generated derivative Tc (M2-IRES-R co-expresses rtTA-M2 TetR-KRAB from single vector. We show compared M2-IRES-R leads greater TRE-mediated transcription presence doxycycline (Dox) yet similar basal Dox. Furthermore, also less systems. Taken together, our results suggest enables regulate highly induce other