Chondrogenic derivatives of embryonic stem cells seeded into 3D polycaprolactone scaffolds generated cartilage tissue in vivo.
作者： Christopher Fecek , Donggang Yao , Ardit Kaçorri , Adrian Vasquez , Samina Iqbal
DOI: 10.1089/TEN.TEA.2007.0293
关键词:
摘要: In spite of recent scientific advances, treatment and repair cartilage using tissue engineering techniques remains challenging. The major constraint is the limited proliferative capacity mature autologous chondrocytes used in approach. This problem can be addressed by stem cells, which self-renew with greater potential. Cartilage adult mesenchymal cells derived from bone marrows has met success. this study we explored generation embryonic (ESCs). ESCs were induced to differentiate into chondroprogenitors, capable proliferating subsequently differentiating cartilage-producing cells. chondrogenic expressed chondrocyte-specific markers deposited extracellular matrix proteins, proteoglycans. ESC-derived polycaprolactone scaffolds seeded these implanted mice (129 SvImJ) generated vivo. Postimplant analysis retrieved tissues demonstrated cartilage-like formation 3-4 weeks. also marker collagen II. These findings suggest that for cultivation tissues.
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