Production of a monoclonal antibody to the arylphorin of Heliothis zea
作者： Cynthia J. Lenz , Matthew H. Greenstone
关键词:
摘要: A species-specific monoclonal antibody was produced to whole plasma of fifth instar larvae the corn earworm, Heliothis zea (Boddie) (Lepid0ptera:Noctuidae). This did not cross-react with proteins from any other lepidopteran tested, including spp. The antigen recognized by this characterized and found be arylphorin, a prominent larval storage protein. conclusion based on electrophoretic as well immunological characteristics antigen. Polyacrylamide gel electrophoresis indicated that had an apparent native molecular weight 460,000, it composed subunits having weights 76,000. isoelectric point 5.9, some microheterogeneity being seen. Western blotting arylphorin against clearly identified arylphorin. protein in egg homogenates or early plasma, but present large quantities pupal and, at trace levels, adult homogenates. efficient production selection hybridomas producing antibodies specific H. using unfractionated immunogen illustrates fact technology can produce highly crude preparations. We discuss tradeoffs one must accept when choosing strategy over purified immunogens.
sci-hub.se 参考文章(21)
David B. Hogg, Erik V. Nordheim, Age-specific survivorship analysis of Heliothis spp. Populations on cotton Population Ecology. ,vol. 25, pp. 280- 297 ,(1983) , 10.1007/BF02515604
G. Galfrè, C. Milstein, [1] Preparation of monoclonal antibodies: Strategies and procedures Methods in Enzymology. ,vol. 73, pp. 3- 46 ,(1981) , 10.1016/0076-6879(81)73054-4
Carlo M. Ignoffo, The nuclear-polyhedrosis virus of Heliothis zea (Boddie) and Heliothis virescens (Fabricius): II. Biology and propagation of diet-reared Heliothis Journal of Invertebrate Pathology. ,vol. 7, pp. 217- 226 ,(1965) , 10.1016/0022-2011(65)90041-8
Mats Carlsson, Anders Hedin, Mats Inganäs, Bengt Härfast, Fred Blomberg, Purification of in vitro produced mouse monoclonal antibodies. A two-step procedure utilizing cation exchange chromatography and gel filtration Journal of Immunological Methods. ,vol. 79, pp. 89- 98 ,(1985) , 10.1016/0022-1759(85)90395-3
Norbert H. Haunerland, William S. Bowers, Arylphorin from the corn earworm, Heliothis zea Insect Biochemistry. ,vol. 16, pp. 617- 625 ,(1986) , 10.1016/0020-1790(86)90004-1
Matthew H. Greenstone, An enzyme-linked immunosorbent assay for the Amblyospora sp. of Culex salinarius (Microspora: Amblyosporidae) Journal of Invertebrate Pathology. ,vol. 41, pp. 250- 255 ,(1983) , 10.1016/0022-2011(83)90225-2
Marco Bestagno, Antonella Cerino, Silvano Riva, Giulia C.B. Astaldi Ricotti, Improvements of Western blotting to detect monoclonal antibodies Biochemical and Biophysical Research Communications. ,vol. 146, pp. 1509- 1514 ,(1987) , 10.1016/0006-291X(87)90820-5
K.B. Elkon, Isoelectric focusing of human IgA and secretory proteins using thin layer agarose gels and nitrocellulose capillary blotting. Journal of Immunological Methods. ,vol. 66, pp. 313- 321 ,(1984) , 10.1016/0022-1759(84)90343-0
Michael Ma, Perry B. Newton, Gong He, Thomas J. Kelly, H.T. Hsu, Edward P. Masler, Alexej B. Borkovec, Development of monoclonal antibodies for monitoring Aedes atropalpus vitellogenesis Journal of Insect Physiology. ,vol. 30, pp. 529- 536 ,(1984) , 10.1016/0022-1910(84)90079-9
C.-P. LIN, T. AN CHEN, Monoclonal antibodies against the aster yellows agent. Science. ,vol. 227, pp. 1233- 1235 ,(1985) , 10.1126/SCIENCE.227.4691.1233