Emerging role of surface plasmon resonance in fragment-based drug discovery

摘要: Surface plasmon resonance (SPR) offers a method of biophysical fragment screening that is fast, efficient, cost effective and accurate. SPR increasingly being adopted as secondary assay to validate hits. Recently, technical advances have resulted in the emergence primary methodology for fragment-based drug discovery. Moreover, biosensor assays can be developed wide range proteins, including membrane such G-protein-coupled receptors. In this review, we discuss advantages limitations experimental consideration reducing false positive negative rates minimum. We how ligand efficiency used both eliminate positives understand which fragments library may source negatives.