Carbachol-induced colonic mucus formation requires transport via NKCC1, K + channels and CFTR

摘要: The colonic mucosa protects itself from the luminal content by secreting mucus that keeps bacteria at a distance epithelium. For this barrier to be effective, has constantly replenished which involves exocytosis and expansion of secreted mucins. Mechanisms involved in regulation are poorly understood, aim study was investigate whether epithelial anion secretion regulates formation colon. muscarinic agonist carbachol used induce parallel anions mucus, using established inhibitors ion transport, we studied how inhibition transport affected mouse Anion mucin were measured changes membrane current capacitance, respectively. Mucus thickness measurements determine effect on growth. results showed carbachol-induced increase dependent NKCC1 co-transport, basolateral K+ channels Cftr activity. In contrast, capacitance partially channel activity, but did not require Carbachol also induced an inhibited blocker bumetanide. However, mice lacked functional respond with thickness, suggesting requires conclusion, these findings suggest affecting both molecules.