Calcium-activated Chloride Channel Regulator 1 (CLCA1) Controls Mucus Expansion in Colon by Proteolytic Activity

摘要: Many epithelial surfaces of the body are covered with protective mucus, and disrupted mucus homeostasis is coupled to diseases such as ulcerative colitis, helminth infection, cystic fibrosis, chronic obstructive lung disease. However, little known how a balanced system maintained. By investigating involvement proteases in colonic dynamics we identified metalloprotease activity be key contributor expansion. The effect was mediated by calcium-activated chloride channel regulator 1 (CLCA1) application recombinant CLCA1 on intestinal freshly dissected tissue resulted increased thickness independently ion secretion, but dependent its metallohydrolase activity. Further, modulated both human mouse, knock-out mice compensated for cysteine proteases. Our results suggest that involved facilitating processing removal prevent stagnation. In light our findings, future studies investigate if upregulation associated accumulation could facilitate an attempt maintain homeostasis.