The Goblet Cell Protein Clca1 (Alias mClca3 or Gob-5) Is Not Required for Intestinal Mucus Synthesis, Structure and Barrier Function in Naive or DSS-Challenged Mice
作者： Nancy A. Erickson , Elisabeth E. L. Nyström , Lars Mundhenk , Liisa Arike , Rainer Glauben
DOI: 10.1371/JOURNAL.PONE.0131991
关键词:
摘要: The secreted, goblet cell-derived protein Clca1 (chloride channel regulator, calcium-activated-1) has been linked to diseases with mucus overproduction, including asthma and cystic fibrosis. In the intestine is found in an abundance expression pattern similar Muc2, major structural component. We hypothesized that required for synthesis, structure or barrier function of intestinal therefore compared wild type Clca1-deficient mice under naive at various time points DSS (dextran sodium sulfate)-challenged conditions. phenotype was systematically characterized by assessment composition using proteomics, immunofluorescence analysis selected mucin genes on mRNA level. Mucus integrity assessed in-vivo bacterial penetration into translocation sentinel organs combined fecal microbiota ex-vivo penetrability beads. All these assays revealed no relevant differences between steady state DSS-challenged conditions mouse colon. not murine
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Magali Agnel, Thierry Vermat, Jean-Michel Culouscou, Identification of three novel members of the calcium‐dependent chloride channel (CaCC) family predominantly expressed in the digestive tract and trachea1 FEBS Letters. ,vol. 455, pp. 295- 301 ,(1999) , 10.1016/S0014-5793(99)00891-1
L Kobzik, J J Godleski, S Huang, J D Paulauskis, Expression of macrophage inflammatory protein-2 and KC mRNA in pulmonary inflammation. American Journal of Pathology. ,vol. 141, pp. 981- 988 ,(1992)
Mohammed Al-Jumaily, Alexei Kozlenkov, Ilana Mechaly, Agnes Fichard, Valerie Matha, Frederique Scamps, Jean Valmier, Patrick Carroll, Expression of three distinct families of calcium-activated chloride channel genes in the mouse dorsal root ganglion Neuroscience Bulletin. ,vol. 23, pp. 293- 299 ,(2007) , 10.1007/S12264-007-0044-8
Jean-Luc Desseyn, Anne Laine, Characterization of mouse muc6 and evidence of conservation of the gel-forming mucin gene cluster between human and mouse. Genomics. ,vol. 81, pp. 433- 436 ,(2003) , 10.1016/S0888-7543(03)00036-3
Isao Okayasu, Shigeru Hatakeyama, Masahiro Yamada, Toshifumi Ohkusa, Yoshio Inagaki, Rintaro Nakaya, A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice Gastroenterology. ,vol. 98, pp. 694- 702 ,(1990) , 10.1016/0016-5085(90)90290-H
Bo Yang, Lin Cao, Jiaen Liu, Yanjie Xu, Gillian Milne, Wanhei Chan, Steven D. Heys, Colin D. McCaig, Jin Pu, Low expression of chloride channel accessory 1 predicts a poor prognosis in colorectal cancer Cancer. ,vol. 121, pp. 1570- 1580 ,(2015) , 10.1002/CNCR.29235
Maria Van der Sluis, Barbara A.E. De Koning, Adrianus C.J.M. De Bruijn, Anna Velcich, Jules P.P. Meijerink, Johannes B. Van Goudoever, Hans A. Büller, Jan Dekker, Isabelle Van Seuningen, Ingrid B. Renes, Alexandra W.C. Einerhand, Muc2-deficient mice spontaneously develop colitis, indicating that MUC2 is critical for colonic protection Gastroenterology. ,vol. 131, pp. 117- 129 ,(2006) , 10.1053/J.GASTRO.2006.04.020
Sumanta Mukherjee, Dennis M. Lindell, Aaron A. Berlin, Susan B. Morris, Thomas P. Shanley, Marc B. Hershenson, Nicholas W. Lukacs, IL-17–Induced Pulmonary Pathogenesis during Respiratory Viral Infection and Exacerbation of Allergic Disease The American Journal of Pathology. ,vol. 179, pp. 248- 258 ,(2011) , 10.1016/J.AJPATH.2011.03.003
Krzysztof Pawłowski, Matti Lepistö, Nina Meinander, Ulf Sivars, Mikael Varga, Elisabet Wieslander, Novel conserved hydrolase domain in the CLCA family of alleged calcium-activated chloride channels. Proteins. ,vol. 63, pp. 424- 439 ,(2006) , 10.1002/PROT.20887
Malin EV Johansson, Daniel Ambort, Thaher Pelaseyed, André Schütte, Jenny K Gustafsson, Anna Ermund, Durai B Subramani, Jessica M Holmén-Larsson, Kristina A Thomsson, Joakim H Bergström, Sjoerd van der Post, Ana M Rodriguez-Pineiro, Henrik Sjövall, Malin Bäckström, Gunnar C Hansson, None, Composition and functional role of the mucus layers in the intestine Cellular and Molecular Life Sciences. ,vol. 68, pp. 3635- 3641 ,(2011) , 10.1007/S00018-011-0822-3