The Effect of Mechanical Stimulation on Mineralization in Differentiating Osteoblasts in Collagen-I Scaffolds

摘要: Developing a viable and functional bone scaffold in vitro that is capable of surviving bearing mechanical load vivo requires an understanding the cell biology osteoprogenitor cells, particularly how they are influenced by stimulation during differentiation maturation. In this study, was applied using modified FlexCell plate to impart confined compression collagen-I scaffolds seeded with undifferentiated murine embryonic stem cells. The activity, presence, expression osteoblast-cadherin (OB-Cad) connexin-43, as well various pluripotent osteogenic markers were examined at 5–30 days cells stimulated differentiate osteoblasts without load. Fluorescence recovery after photobleaching, immunofluorescence, viability, von Kossa, real-time polymerase chain reaction assessments revealed prestimulation cell-seeded altered OB-Cad and...