作者: S Li , , X Jia , VC Duance , EJ Blain
DOI: 10.22203/ECM.V021A38
关键词:
摘要: It is still relatively unclear how intervertebral disc (IVD) cells sense a mechanical stimulus and convert this signal into biochemical response. Previous studies demonstrated that the cytoskeletal elements are mechano-responsive in many cell types may contribute to mechano-signalling pathways. The objective of study was determine response from outer annulus fibrosus (OAF) physiological levels cyclic tensile strain; further, nucleus pulposus (NP) were also subjected an identical loading regime compare biological responses across IVD populations. We determined whether organisation expression major their associated accessory proteins responsive stimulation these cells, changes correlated with either catabolic or anabolic phenotype. OAF NP immature bovine seeded onto Flexcell® type I collagen coated plates. Cells strain (10 %, 1 Hz) for 60 minutes. Post-loading, processed immunofluorescence microscopy, RNA extracted quantitative PCR protein Western blotting analysis. F-actin reorganisation evident induced β-actin at transcriptional translational level cells. β-tubulin mRNA synthesis increased strained but vimentin significantly inhibited. Cytoskeletal element less Tensile differentially regulated extracellular matrix (ECM)-degrading enzymes’ Strain II transcription had no effect on any other genes analysed. induces different mechano-responses and/or markers metabolism. Differential mechano-regulation ECM components populations reflects respective environments situ.