3,3′-Diindolylmethane nanoencapsulation improves its antinociceptive action: Physicochemical and behavioral studies

摘要: Abstract This study aimed to characterize the physicochemical properties of 3,3′-diindolylmethane (DIM)-loaded nanocapsules (NCs) as well antinociceptive effect using distinct animal models (hot plate test, formalin-induced nociception and complete Freud’s adjuvant induced paw inflammation). The DIM-loaded NCs (composed by primula oil ethylcellulose) were characterized differential scanning calorimetry, thermogravimetric analysis, Fourier-transformed infrared spectroscopy, X-ray diffractometry electron microscopy. characterization demonstrated that DIM could be molecularly dispersed into NCs, whose size was nanometric with a spherical shape. An improvement in thermal stability achieved its encapsulation there no interactions among formula components. For nociceptive evaluation, male adult Swiss mice pretreated or free intragastric route at dose 10 mg/Kg (time-response curve), 5 2.5 mg/Kg (dose-response curve). behavioral tests performed over an experimental period 0.5–8 h. Both nanoencapsulated reduced mechanical hypernociception CFA, mitigated behavior neurogenic inflammatory pain increased withdrawal latency assessed hot-plate test. Importantly, nanoencapsulation promoted rapid initiation prolonged bioactive action (up 8 h) effective comparison form. In summary, this reported had adequate size, different models, suggesting formulation may possible therapeutic alternative management inflammatory-related pathologies.