Suspension culture of Drosophila cells employing a gyratory shaker.

摘要: To develop a method for culturing large number of small-scale suspension cultures ofDrosophila melanogaster cells simultaneously, basic conditions were studied using cell line GM2 and gyratory shaker. Under gyration at more than 180 rpm, majority (>80%) the still remained as grew normally. Lower speed caused adhesion to substratum. Furthermore, size culture vessels was found affect pattern growth. Five- or 10-ml Erlenmeyer flasks gave satisfactory results, but growth curves in 30-ml differed from flask saturation level lower. Besides, latter case quite different depending on volume medium. A preliminary experiment showed that type might curve. Initial densities has be 6×104 per ml. resulted longer doubling time no increase number. Therefore following are recommended standard ofD. cell, GM2: gyration, rpm; vessel, 5- certain type; initial density, 1 5×105 Both D20 modified Schneider’s medium could utilized