Differential expression of MART-1, tyrosinase, and SM5-1 in primary and metastatic melanoma.
作者： Susanne Reinke , Peter K??niger , Gunda Herberth , Heike Audring , Hao Wang
DOI: 10.1097/01.DAD.0000180076.17932.EE
关键词:
摘要: The new monoclonal antibody SM5-1 has been shown to have significant advantages in immunohistochemistry of melanoma over currently used antibodies such as HMB-45 or anti-S100. In this study we compared the immunohistological staining pattern with that more recently described A103 (anti-MART-1) and T311 (anti-Tyrosinase) 344 paraffin-embedded specimens, consisting 101 primary melanomas (77 SSM, 16 NM, 6 ALM, 2 LMM) 243 metastases. overall reactivity for all specimens was 92% (318/344) 83% (285/344) MART-1 71% (245/344) Tyrosinase. Staining metastases found 91% (222/243), but only 77% (187/243) 63% (154/243) T311, respectively. homogenous 196 (80%) metastatic lesions showing 50% positively stained cells within lesions, whereas did so 141 (58%) 117 (48%) lesions. With regard intensity SM5-1, 157 (64%) showed a strong very intensity, 85 (35%) 70 (29%) percentage positivity correlated well because from 58 positive 55 than 75% lesion. Importantly, 52 56 MART-1-negative 81 89 Tyrosinase-negative were SM5-1. Thirty-eight (15.6%) negative both T311. Of those, 35 (92.1%) demonstrating value identifying melanoma-associated antigen-negative We conclude could be melanoma.
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