Ig allotypic inclusion does not prevent B cell development or response.

摘要: B cells expressing two different Igκ L chains (allotype included) have been occasionally observed. To determine frequency and function of these cells, we analyzed gene-targeted mice that carry a human mouse Igk C region genes. Using methodologies, found distinct κ-chains were 1.4–3% all they present in the follicular, marginal zone, B1 mature cell subsets. When stimulated vitro with anti-IgM, dual κ surface-positive underwent activation manifested proliferation and/or up-regulation markers similar to single κ-expressing cells. Yet, when activated by divalent reagents bound only one κ-chains, responded suboptimally vitro, most likely because reduced Ag receptor cross-linking. Nonetheless, participated SRBC-specific immune response vivo. Finally, Ig allotype-included coexpress autoreactive nonautoreactive receptors also capable responses following BCR aggregation. In summary, our studies demonstrate are population responsive stimulation both Moreover, anti-IgM was observed coexpressing Abs, suggest potential role physiological pathological responses.