A dynein-like protein from brain

摘要: Dynein forms the arms on microtubule doublets of cilia and flagella [l-3] , has a monomeric sedimentation coefficient approximately 14 S [l-S] separates SDSpolyacrylamide gel electrophoresis into two high molecular weight components [S, 61. In presence Mg2+ or Ca2+, dynein catalyzes hydrolysis ATP [3-5,7]. The bending is thought to result from sliding adjacent [8, 91, affected by dynein-dependent [lo, 111. We have detected brain protein with physical properties similar those one Spisu2a sperm tail dyneins, although isolated little no ATPase activity. This dynein-like associated microtubules reassembled in vitro.