Identification of Genes Up-Regulated in Urothelial Tumors: The 67-kd Laminin Receptor and Tumor-Associated Trypsin Inhibitor

摘要: Studies investigating changes in gene expression urothelial carcinoma have generally compared tumors of different stages and grades but comparisons between low-grade, noninvasive normal urothelium are needed to identify genes involved early tumor development. We isolated the from a low-grade corresponding mucosa by laser capture microdissection on frozen sections. The RNA extracted was amplified generate suppressive subtractive cDNA libraries. Random sequencing clones identified ∼100 unique species. Of these 83% were known genes, 15% had homology with an unknown function humans, 2% did not show any published sequence. Two 67-kd laminin receptor (67LR) tumor-associated trypsin inhibitor (TATI), previously been associated metastatic progression many types, although 67LR has investigated tumors. Immunolabeling original tissue antibodies against two confirmed overexpression, validating our strategy: expressed present tumor, whereas TATI confined umbrella cells urothelium, extended all cell layers tumor. both markers further separate series grades. more consistently overexpressed than stages. Levels secreted significantly higher urine samples patients controls. Our strategy, combining library construction, that may be phases development rather disease progression, highlighting importance comparing just