Heterochromatin composition and function in Drosophila

摘要: A large variety of proteins bind to specific parts the genome regulate gene expression, DNA replication, and chromatin structure. DamID is a powerful method used map genomic interaction sites these in vivo. It based on fusing protein interest E. coli adenine methyltransferase (dam). Expression this fusion vivo leads preferential methylation adenines surrounding native binding dam partner. Because does not occur endogenously most eukaryotes, it provides unique tag mark sites. The adenine-methylated fragments are isolated by selective PCR amplification can be identified microarray hybridization. We others have successfully applied genome-wide identification several transcription factors other chromatin-associated proteins. Here technology discussed detail, step-by-step experimental protocol provided for use Drosophila cell lines.