Volatile ketone formation in bacteria: release of 3-oxopentanoate by soil pseudomonads during growth on heptanoate.

作者: Michael G. Matiasek , Keka Choudhury , Michele Nemecek-Marshall , Ray Fall

DOI: 10.1007/S002840110217

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摘要: During enrichments to search for soil bacteria that form the volatile ketones, acetone and butanone, we isolated pure cultures of Pseudomonas aureofaciens, P. fluorescens, putida excrete β-keto-acid, 3-oxopentanoate (3-OPA), when grown on heptanoic acid as carbon source. Analysis 3-OPA used enzymatic decarboxylation by acetoacetate decarboxylase yield which was detected headspace gas chromatography. The formation strongly dependent concentration, level oxygen, state growth, not seen with even-chain or other odd-chain fatty acids. Uptake during stationary phase growth is probably related polyhydroxyalkanoate (PHA) in these isolates. A model release proposed.

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