Labeling reactions applicable to chromatography and electrophoresis of minute amounts of proteins

摘要: Chromatography and electrophoresis have become extremely valuable important methods for the separation, purification, detection analysis of biopolymers HPLC/HPCE may premier, preferable approaches both qualitative quantitative analyses most proteins, especially from recombinant materials. This includes smaller peptides, polypeptides, antibodies all types protein or antibody-conjugates (antibody-enzyme, protein-fluorescent probe, antibody-drug so forth). entire Topical Issue Journal emphasizes application chromatography to analysis. particular review deals with selective tagging labeling proteins at trace (minute) levels, again using either electrophoresis, emphasis on modern approaches. We discuss here pre- post-column reagents, techniques realizing antibodies, applicable preconcentration in HPLC HPCE areas general, improving (lowering) limits utilizing chemical physical derivatization and/or techniques. There are really two major goals emphases that which follows: (1) prior after (2) levels improved separation-detection lowered limits. a large number specific references related post-column/capillary derivatizations as well detectability by, example, analyte solid-phase extractor membrane support, capillary isotachophoresis other methods. Selective reactions refers ability tag (e.g. reactive amino sites) controlled manner, products having same tags very site sites. The species, locations protein. can also refer idea sample only single, available sites, homogeneously.