Analysis of phosphorylation of tau with antibodies specific for phosphorylation sites.
作者： Koichi Ishiguro , Kazuki Sato , Masako Takamatsu , Jungmi Park , Tsuneko Uchida
DOI: 10.1016/0304-3940(95)12206-0
关键词:
摘要: Abstract Previously, we determined sites of tau protein phosphorylation by kinase (TPK) I/glycogen synthase 3β (GSK-3β) and TPKII/(cyclin-dependent 5 (CDK5) + p23). We prepared antibodies specific for these phosphorylated TPKI TPKII, using chemically synthesized phosphopeptides as antigens. Each antibody specifically reacts with each site. With antibodies, it was confirmed that TPKII are responsible sites, reported previously, except Ser404 is also weakly alone. It observed TPKII-phosphorylation enhances TPKI-phosphorylation. These results indicate useful tools investigation the TPKII.
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