Enhanced serum response element binding activity correlates with down-regulation of c-fos mRNA expression in the rat brain following repeated cortical lesions.

摘要: Repeated lesions of rat cerebral cortex result in transient peaks the level c-fos transcript, but after second lesion, this peak is substantially diminished. Using lesion paradigm, we have analyzed participation promoter elements SRE and DSE regulation transcription. Following a single SRE/DSE binding activity peaked at 2 h, subsequent to maximal levels mRNA parallel c-Fos protein. After (reinduction), 4 h following initial only 30 min was significantly higher than first lesion. Once again, occurred expression with protein expression. These results suggested that participated induction down-regulation transcription vivo possible involvement Fos its own regulation. The ability Fos/Fra proteins participate transcriptional complex confirmed gel-shift experiments an AP-1 element, biphasic trend observed. Supershift were performed directly determine whether participating and/or complexes. No alterations position or intensity shifted band observed using antiserum suggesting could be involved through mechanisms other direct complex.