Poly(A)+ RNA from Tetrahymena: stimulation of protein synthesis in vitro.
作者： M. LYNN MYHAL , LINDA A. HUFNAGEL
DOI: 10.1111/J.1550-7408.1979.TB04218.X
关键词:
摘要: Cell-free synthesis of high molecular weight polypeptides, programmed by RNA from Tetrahymena pyriformis strain W is reported, and methods for preparation the are described. The was extracted SDS-phenol-chloroform-isoamyl alcohol technic. bulk ribosomal on sucrose gradients peaked at approximately 17S 25S. After heat denaturation all 25S converted to 17S, indicating presence hidden breaks, possibly result nuclease activity during extraction. Nevertheless, when poly(A) +/- collected using oligo-(dT)-cellulose column chromatography, it promoted a 15-fold increase in incorporation [35S] methionine into TCA-precipitable material. Slab-gel electrophoresis autoradiography product revealed 12 different major varying 28,000 65,000 Daltons. A method translatable will make possible comparison messenger RNAs associated with specific cell structures developmental events.
sci-hub.se 参考文章(12)
Stephen M. Boyle, Paul S. Cohen, Putrescine-mediated Degradation of Ribonucleic Acid in Chloramphenicol-treated Escherichia coli1 Journal of Bacteriology. ,vol. 96, pp. 1266- 1272 ,(1968) , 10.1128/JB.96.4.1266-1272.1968
Martin A. Gorovsky, John Woodard, STUDIES ON NUCLEAR STRUCTURE AND FUNCTION IN TETRAHYMENA PYRIFORMIS: I. RNA Synthesis in Macro- and Micronuclei. Journal of Cell Biology. ,vol. 42, pp. 673- 682 ,(1969) , 10.1083/JCB.42.3.673
Werner A. ECKERT, Walter KAFFENBERGER, Georg KROHNE, Werner W. FRANKE, Introduction of Hidden Breaks during rRNA Maturation and Ageing in Tetrahymena pyriformis FEBS Journal. ,vol. 87, pp. 607- 616 ,(1978) , 10.1111/J.1432-1033.1978.TB12413.X
Donald P. Weeks, Philip S. Collis, Induction of microtubule protein synthesis in Chlamydomonas reinhardi during flagellar regeneration Cell. ,vol. 9, pp. 15- 27 ,(1976) , 10.1016/0092-8674(76)90048-9
A. Ron, O. Horovitz, I. Sarov, Fractionation of RNA from Tetrahymena by Affinity Chromatography on Poly-U-Sepharose* Journal of Molecular Evolution. ,vol. 8, pp. 137- 142 ,(1976) , 10.1007/BF01739099
C.J. Bostock, D.M. Prescott, M. Lauth, Lability of 26 S ribosomal RNA in Tetrahymena pyriformis. Experimental Cell Research. ,vol. 66, pp. 260- 262 ,(1971) , 10.1016/S0014-4827(71)80038-1
M.-C. Yao, A. R. Kimmel, M. A. Gorovsky, A small number of cistrons for ribosomal RNA in the germinal nucleus of a eukaryote, Tetrahymena pyriformis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 71, pp. 3082- 3086 ,(1974) , 10.1073/PNAS.71.8.3082
RUTH ROMAN, JOHN D. BROOKER, SAMARENDRA N. SEAL, ABRAHAM MARCUS, Inhibition of the transition of a 40S ribosome–Met-tRNA i Met complex to an 80S ribosome–Met-tRNA i Met complex by 7-methylguanosine-5′-phosphate Nature. ,vol. 260, pp. 359- 360 ,(1976) , 10.1038/260359A0
Allan Spradling, Helen Hui, Sheldon Penman, Two very different components of messenger RNA in an insect cell line Cell. ,vol. 4, pp. 131- 137 ,(1975) , 10.1016/0092-8674(75)90119-1
H. Aviv, P. Leder, Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose Proceedings of the National Academy of Sciences of the United States of America. ,vol. 69, pp. 1408- 1412 ,(1972) , 10.1073/PNAS.69.6.1408