Regulation of Dynamic Changes in Cell Wall Polysaccharides

摘要: ABSTRACT When tobacco BY-2 suspension cells are cultured in the presence of a cellulose synthesis inhibitor, 1 μM 2,4-dichrolobenzonitrile (DCB), swell, grow slowly but survive. The cell walls consist 66% pectin, 30% hemicellulose and only 3% cellulose. DCB treatment did not affect mRNA level CesA (cellulose synthase). Electron micrography with immunogold labeling technique demonstrated that DCB-habituated accumulated protein cytoplasm more than normal cells. probably inhibits after transcription CesA. treatment, however, decreased EXGT (endoxyloglucan transferase). Treatment cellulase also EXGT, suggesting defect or weakening regulates expression. Cellulase cellulase-induced decrease expression was partly overcome by addition osmoticum (0.45 M mannitol) to medium, implying turgor change stretch plasmamembrane is involved regulation mechanism. Pretreatment actinomycin D cancelled indicating needs function another gene. These results suggest (1) plant can monitor alternations wall architecture sensing as yeast bacteria, (2) regulated some gene mechanism, (3) rather insensitive