Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense.
作者： T. Baltz , D. Baltz , C. Giroud , J. Crockett
DOI: 10.1002/J.1460-2075.1985.TB03772.X
关键词:
摘要: A semi-defined medium for the cultivation of bloodstream forms African trypanosome brucei subgroup was developed. Out 14 different strains tested, 10 could be cultured including Trypanosoma brucei, T. equiperdum, evansi, rhodesiense and gambiense. The presence a reducing agent (2-mercaptoethanol or thioglycerol) found to essential growth. standard consisted Hepes buffered minimum with Earle's salts supplemented 0.2 mM 2-mercaptoethanol, 2 pyruvate 10% inactivated serum either from rabbit (T. evansi rhodesiense) human gambiense). Although general defined long-term maintenance cultures, initiation culture nevertheless required particular conditions strains. trypanosomes had all characteristics in vivo including: morphology, infectivity, antigenic variation glucose metabolism.
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sci-hub.se 参考文章(15)
H Hirumi, J. Doyle, K Hirumi, African trypanosomes: cultivation of animal-infective Trypanosoma brucei in vitro. Science. ,vol. 196, pp. 992- 994 ,(1977) , 10.1126/SCIENCE.558652
A. Balber, Primary murine bone marrow cultures support continuous growth of infectious human trypanosomes. Science. ,vol. 220, pp. 421- 423 ,(1983) , 10.1126/SCIENCE.6836284
G. Riou, Th. Baltz, M. Gabillot, R. Pautrizel, Absence of kinetoplast DNA in a late antigenic variant of Trypanosoma equiperdum. Molecular and Biochemical Parasitology. ,vol. 1, pp. 97- 105 ,(1980) , 10.1016/0166-6851(80)90004-3
R. BRUN, L. JENNI, M. SCHÖNENBERGER, K.-F. SCHELL, In vitro cultivation of bloodstream forms of Trypanosoma brucei, T. rhodesiense, and T. gambiense. Journal of Eukaryotic Microbiology. ,vol. 28, pp. 470- 479 ,(1981) , 10.1111/J.1550-7408.1981.TB05322.X
Wallace R. Fish, Douglas L. Looker, J. Joseph Marr, Randolph L. Berens, Purine metabolism in the bloodstream forms of Trypanosoma gambiense and Trypanosoma rhodesiense. Biochimica et Biophysica Acta. ,vol. 719, pp. 223- 231 ,(1982) , 10.1016/0304-4165(82)90092-7
W.H.R. Lumsden, W.J. Herbert, Pedigrees of the Edinburgh Trypanosoma (Trypanozoon) antigenic types (ETat). Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 69, pp. 205- 208 ,(1975) , 10.1016/0035-9203(75)90156-X
Keith Vickerman, Antigenic variation in trypanosomes Nature. ,vol. 273, pp. 613- 617 ,(1978) , 10.1038/273613A0
M. R. Rifkin, Identification of the trypanocidal factor in normal human serum: high density lipoprotein. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 3450- 3454 ,(1978) , 10.1073/PNAS.75.7.3450
S R Meshnick, S H Blobstein, R W Grady, A Cerami, An approach to the development of new drugs for African trypanosomiasis. Journal of Experimental Medicine. ,vol. 148, pp. 569- 579 ,(1978) , 10.1084/JEM.148.2.569
B A Arrick, O W Griffith, A Cerami, Inhibition of glutathione synthesis as a chemotherapeutic strategy for trypanosomiasis. Journal of Experimental Medicine. ,vol. 153, pp. 720- 725 ,(1981) , 10.1084/JEM.153.3.720