Solid-phase detection of RNA using bispyrene-modified RNA probe

摘要: Bispyrene modified 2'-O-methyl oligoRNA (OMUpy2), which is a useful fluorescent probe for specific RNA detection, was immobilized on glass substrate via various linker molecules to develop more convenient detection chip gene expression study. As the fluorescence intensity of OMUpy2 enhanced greatly when it hybridized with its complementary RNA, expected that by OMUpy2-immobilized (RNA-chip) required neither labels target nucleic acid nor washing protocol after hybridization. molecules, oligo(dT), agarose, and poly(ethylene glycol) (PEG) were chosen, from measured microscope. Results indicated RNA-chip able detect oligoribonucleotide (cORN) without labeling protocols. Furthermore, background emission reduced as length increased. In case PEG linker, 25-fold enhancement observed upon addition cORN.