Injectable tissue adhesive composite hydrogel with fibroblasts for treating skin defects

摘要: In this work, an injectable composite hydrogel was synthesized via a unique way of crosslinking glycol chitosan (GC) with silica nano-particles (SiNP) through non-chemical interactions, and then applied as kind wound dressing. Gelation achieved the incorporation SiNPs GC segments in aqueous solution, therefore strictly confining movement solubilized polymer chains. Rheology tests showed that sol–gel transition moduli were influenced by composition two components, size conformation polymers. Using such strategy, tissue adhesion properties well-preserved GC/SiNP it gains gluey toward biological tissues demonstrated pieces mouse skin, obtaining lap-shear stretching force ca. 90 kPa. This characteristic, together injectability, allowed to be administrated directly on site fill area. Meanwhile, also works carrier protein cells. The situ encapsulation fibroblasts enabled promising used for treating full-thickness skin defects model, resulting favorable growth hair follicles microvessels, hence reducing risk scar formation.