Biocontrol of Isolated Cronobacter spp. (Enterobacter sakazakii) by Heat, Sanitizer, and Antibiotic

摘要: Tolerance against heat, sanitizers, and antibiotics of 112 Cronobacter isolates classified by desiccation was determined to permit effective biocontrol in powdered foods. The were into three groups: dry-tolerant (n=37), dry-sensitive (n=7), dry-intermediate (n=68). strains that highly tolerant drying also showed high heat tolerance they seemed have after dry stress foods like infant formula. Sodium hypochlorite benzalkonium chloride concentrations necessary achieve a 5-log reduction viable counts (CFU/mL) 15-25 ppm 5-15 ppm, respectively. However, there little difference the efficacy these sanitizers between -tolerant for planktonic cells suspended 3% albumin. minimal inhibition concentration (MIC) -lactam ampicillin 64-128 90% strains. consistently sensitive kanamycin naldixic acid (MIC=4 ppm). Dry-tolerant displayed more antibiotic resistance than results indicate often possess resistance, indicated need potent sterilization treatments