Characterization of the salivary microbiome in patients with pancreatic cancer

摘要: Clinical manifestations of pancreatic cancer often do not occur until the has undergone metastasis, resulting in a very low survival rate. In this study, we investigated whether salivary bacterial profiles might provide useful biomarkers for early detection cancer. Using high-throughput sequencing small subunit ribosomal RNA (16S rRNA) gene, characterized microbiota patients with and compared them to healthy other diseases, including disease, non-pancreatic digestive disease/cancer non-digestive disease/cancer. A total 146 were enrolled at UCSD Moores Cancer Center where saliva demographic data collected from each patient. Of these, analyzed microbiome 108 patients: 8 had been diagnosed cancer, 78 diseases 22 classified as non-diseased (healthy) controls. Bacterial 16S rRNA sequences amplified directly DNA extractions subjected (HTS). Several genera differed abundance We found significantly higher ratio Leptotrichia Porphyromonas than or those disease (Kruskal-Wallis Test; P < 0.001). abundances confirmed using real-time qPCR specific primers. Similar previous studies, lower relative Neisseria Aggregatibacter patients, though these results significant 0.05 level (K-W = 0.07 0.09 respectively). However, previously identified biomarkers, e.g., Streptococcus mitis Granulicatella adiacens, different patients. Overall, study supports hypothesis that bacteria are much larger patient studies needed verify their predictive utility.