The key microorganisms for anaerobic degradation of pentachlorophenol in paddy soil as revealed by stable isotope probing

摘要: Pentachlorophenol (PCP) is a common residual persistent pesticide in paddy soil and has resulted harmful effect on ecosystem. The anaerobic microbial transformation of PCP, therefore, been received much attentions, especially the functional communities for reductive transformation. However, key microorganisms PCP mineralization still remain unknown. In this work, DNA-based stable isotope probing (SIP) was applied to explore responsible soil. SIP results indicated that dominant bacteria biodegradation belonged genus Dechloromonas class β-Proteobacteria. addition, increased production 13CH4 13CO2 addition lactate enhanced rate PCP. Two archaea classified as genera Methanosaeta Methanocella Methanobacteria were enriched heavy fraction when with lactate, whereas no detected absence lactate. These findings provide direct evidence species or its breakdown products reveal new insight into linked degradation