作者: José Ramiro Fernandes , Leonor Pereira , Pedro Jorge , Luis Moreira , Helena Gonçalves
DOI: 10.1051/BIOCONF/20150502021
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摘要: The wine sector is a billion euro business and therefore subjected to multiple attempts of fraudulent practices. This requires the development rapid reliable methods detect such situations. Several methodologies have been developed based on chemical profiles wines, but they are limited due environmental conditions that cannot be controlled. use DNA-based detection systems an emergent research field extended wide variety food prod- ucts still most for varietal identification. However these not suitable geographical determination. Soil related fingerprints primary role considering there relationship between elemental compo- sition composition provenance soil. WineBioCode project aiming define best strategy authenticity multidisciplinary approach. Two strategies Real-time PCR label free optical biosensor platform. Both platforms enabled successful identification specific DNA-targets when applied Vitis vinifera L., can throughout grape-wine chain. complementary used in dif- ferent situations, according requirements. evaluation has assessed by strontium 87 Sr/86 Sr isotope ratio determination involving soil vineyards followed its assay samples. results being integrated order establish procedure undertaken fingerprinting, including origin, fulfilling requirements denominations certification.