Replication slippage involves DNA polymerase pausing and dissociation.
作者： Enrique Viguera , Danielle Canceill , S Dusko Ehrlich
关键词:
摘要: Genome rearrangements can take place by a process known as replication slippage or copy-choice recombination. The occurs between repeated sequences in both prokaryotes and eukaryotes, is invoked to explain microsatellite instability, which related several human diseases. We analysed the molecular mechanism of short direct repeats, using vitro single-stranded DNA template that mimics lagging strand synthesis. show involves polymerase pausing, must within repeat, pausing dissociates from DNA. also present evidence that, upon dissociation, only terminal portion newly synthesized separates anneals another repeat. Resumption then completes process.
core.ac.uk
nature.com
sci-hub.se 参考文章(51)
H. Bierne, S.D. Ehrlich, B. Michel, The replication termination signal terB of the Escherichia coli chromosome is a deletion hot spot. The EMBO Journal. ,vol. 10, pp. 2699- 2705 ,(1991) , 10.1002/J.1460-2075.1991.TB07814.X
E. d'Alençon, M. Petranovic, B. Michel, P. Noirot, A. Aucouturier, M. Uzest, S.D. Ehrlich, Copy-choice illegitimate DNA recombination revisited The EMBO Journal. ,vol. 13, pp. 2725- 2734 ,(1994) , 10.1002/J.1460-2075.1994.TB06563.X
C.S. McHenry, DNA polymerase III holoenzyme. Components, structure, and mechanism of a true replicative complex. Journal of Biological Chemistry. ,vol. 266, pp. 19127- 19130 ,(1991) , 10.1016/S0021-9258(18)54967-X
John M Darlow, David R.F Leach, Evidence for two preferred hairpin folding patterns in d(CGG).d(CCG) repeat tracts in vivo. Journal of Molecular Biology. ,vol. 275, pp. 17- 23 ,(1998) , 10.1006/JMBI.1997.1452
P Bedinger, M Munn, B M Alberts, Sequence-specific pausing during in vitro DNA replication on double-stranded DNA templates. Journal of Biological Chemistry. ,vol. 264, pp. 16880- 16886 ,(1989) , 10.1016/S0021-9258(19)84789-0
M O'Donnell, P S Studwell, Total reconstitution of DNA polymerase III holoenzyme reveals dual accessory protein clamps. Journal of Biological Chemistry. ,vol. 265, pp. 1179- 1187 ,(1990) , 10.1016/S0021-9258(19)40175-0
ME O'Donnell, Accessory proteins bind a primed template and mediate rapid cycling of DNA polymerase III holoenzyme from Escherichia coli. Journal of Biological Chemistry. ,vol. 262, pp. 16558- 16565 ,(1987) , 10.1016/S0021-9258(18)49292-7
K.J. Hacker, B.M. Alberts, The rapid dissociation of the T4 DNA polymerase holoenzyme when stopped by a DNA hairpin helix. A model for polymerase release following the termination of each Okazaki fragment. Journal of Biological Chemistry. ,vol. 269, pp. 24221- 24228 ,(1994) , 10.1016/S0021-9258(19)51071-7
M.K. Reddy, S.E. Weitzel, P.H. von Hippel, Processive proofreading is intrinsic to T4 DNA polymerase Journal of Biological Chemistry. ,vol. 267, pp. 14157- 14166 ,(1992) , 10.1016/S0021-9258(19)49692-0
D.T. Weaver, M.L. DePamphilis, Specific sequences in native DNA that arrest synthesis by DNA polymerase alpha. Journal of Biological Chemistry. ,vol. 257, pp. 2075- 2086 ,(1982) , 10.1016/S0021-9258(19)68148-2