Status epilepticus-induced hilar basal dendrites on rodent granule cells contribute to recurrent excitatory circuitry.

摘要: Mossy fiber sprouting into the inner molecular layer of dentate gyrus is an important neuroplastic change found in animal models temporal lobe epilepsy and humans with this type epilepsy. Recently, we reported perforant path stimulation model another for granule cells following seizures: hilar basal dendrites (HBDs). The present study determined whether status epilepticus-induced HBDs on occur pilocarpine these are targeted by mossy fibers. Retrograde transport biocytin its ejection stratum lucidum CA3 was used to label both light electron microscopy. Granule a heterogeneous morphology, including recurrent dendrites, locations outside cell were observed control preparations. Preparations from kainate also showed HBDs. These branched extended hilus shown be 5% pilocarpine-treated rats epilepticus, whereas had virtually none. Electron microscopy determine postsynaptic axon terminals hilus, site where collaterals prevalent. Labeled form asymmetric synapses labeled Also, unlabeled, large boutons presynaptic cells. results indicate that epilepsy, confirm presence model, show new may contribute additional excitatory circuitry