Chromogenic in situ hybridization accurately identifies EGFR amplification in small cell glioblastoma multiforme, a common subtype of primary GBM

摘要: Primary glioblastoma multiforme (GBM) commonly overexpresses the epidermal growth factor receptor (EGFR) gene and its ligand-independent mutant, EGFRvIII. Amplification of EGFR has been implicated in pathogenesis primary GBM, particular small cell phenotype, this finding may contribute to aggressive clinical behavior. Anti-EGFR trials for GBM are being conducted, it would be useful identify a rapid technique determine whether expression phenotype associated with response therapy. In present study we examined 56 cases using chromogenic situ hybridization (CISH). CISH analysis morphology identified 22 (SCGBM) non-small (NSCGBM), 12 mixed phenotype. Fourteen SCGBM (14/22) showed amplification, while only 5 NSCGBM (5/22) amplification. We have therefore used as an efficient, economic reliable means routinely assessing amplification including variant.