Evaluation of NF2 gene deletion in sporadic schwannomas, meningiomas, and ependymomas by chromogenic in situ hybridization

摘要: Fluorescence in situ hybridization, loss of heterozygosity testing, and comparative genomic hybridization have been used to detect NF2 gene alterations both sporadic neurofibromatosis type 2 (NF2)-associated central nervous system tumors. In this study, we performed chromogenic (CISH) immunohistochemistry evaluate for deletion a group meningiomas, schwannomas, ependymomas. Twenty-two tumors, including 9 ependymomas, 10 3 were studied. CISH using the probe polyclonal antibody. Deletion was identified 11 (50%) 60% (6/10) 33% (3/9) 67% (2/3) schwannomas. The remaining cases diploid. Overall, immunoexpression protein observed 50% (11/22) concordance between 73% cases. Our results support previous observations that schwannomas lesser degree, express high incidence deletion, which supports hypothesis plays an important role their tumorigenesis. addition, validated as efficient, economic, reliable method routinely assessing these