Evaluation of epidermal growth factor receptor (EGFR) by chromogenic in situ hybridization (CISH) and immunohistochemistry (IHC) in archival gliomas using bright-field microscopy.

摘要: Overexpression of EGFR secondary to gene amplification is a common feature in primary malignant gliomas. To correctly assess protein and level as possible prognostic predictive markers gliomas, straightforward assays, which can be used routinely the pathology laboratory evaluate status, becomes critical. chromosome 7 aneuploidy was detected 34 formalin-fixed, paraffin-embedded benign gliomas by chromogenic situ hybridization (CISH) using digoxigenin-labeled biotin-labeled centromeric probes. The results were evaluated bright-field microscopy under 40x objective lens. immunohistochemistry (IHC) monoclonal antibody 31G7. Five cases, 3 astrocytoma grade III (33%) 2 glioblastoma multiforme (GBM) (33%), had displayed diaminobenzidine-stained multiple dots suggesting pattern double-minute chromosomes. Chromosome polysomy found 68% 100% GBM, 67% III, 42% II, 50% I, ependymoma, 1 case mixed glioma III. High expression present 62% membrane cytoplasmic staining. All tumors with showed high expression. without observed all grades Simultaneous detection copies or centromere signals along tissue morphology allows us compare CISH easily IHC results. Our show that an objective, practical, accurate assay screen for status