Clinically distinct subgroups of glioblastoma multiforme studied by comparative genomic hybridization.

摘要: Studies investigating genetic alterations potentially constituting prognostic factors in glioblastoma multiforme (GBM) have centered mainly around amplification events. Comparative genomic hybridization (CGH) is a recent molecular cytogenetic technique that allows the detection of chromosomal imbalances and sites tumor DNA prepared from fresh or archival material. A group 94 patients with GBM underwent surgery followed by standard course radiotherapy. Neuroradiologic monitoring gadolinium-enhanced serial magnetic resonance imaging was applied to study radiologically progression-free interval (RPFI) regrowth velocity. These parameters provided clinical estimate postoperative kinetics yielded two clinically distinct groups. The most pronounced cases were selected each group, i.e., those favorable unfavorable prognosis. Two subgroups statistically significant difference RPFI (p < 0.001, Mann-Whitney U test) containing 10 formed : Subgroup (slow kinetics) subgroup B (fast kinetics). For search might be correlated kinetics, we CGH formalin-fixed, paraffin-embedded tissue these 20 patients. Except for autosomes 18 21, all chromosomes involved at least once copy-number aberrations. Events commonly associated GBM, gains chromosome 7, complete partial losses 9p, 10, 22q, not distributed differently between subgroups. following differences noticeable. Gains (including amplifications) 12q14-q21 19 observed more often A. Losses 6q16-qter parts 13, 20, frequent B. significantly shorter without than gene amplification. did differ 7p12 amplification, where epidermal growth factor receptor localized. New tumors revealed 11q13 11q22-q23. Loss restricted bands 10q25-q26 one case. Although karyotypes slow fast tumor-regrowth noted, present reveal any single alteration useful as factor. In particular, data do support assumption suffering events would poorer prognosis others.