HYPERPRODUCTION AND GENE AMPLIFICATION OF THE EPIDERMAL GROWTH FACTOR RECEPTOR IN SQUAMOUS CELL CARCINOMAS
作者： Masakazu Ueda , Nobuyoshi Shimizu , Nobuyoshi Shimizu , Osahiko Abe , John Hunts
DOI: 10.20772/CANCERSCI1985.76.8_663
关键词:
摘要: Human squamous cell carcinoma tissue was screened to determine the epidermal growth factor (EGF) receptor level. In 9 out of 15 cases, increased EGF binding observed relative normal adjacent tissue. two samples (SCE1 and SCL1), amplification gene mRNA level were also observed. other cases (SCE2 SCL3), did not occur. We propose that there is an alternative mechanism for hyperproduction, independent content, active in these tissues. A possible role hyperproduction envisioned human neoplasia.
sci-hub.st 参考文章(10)
John H. Hunts, Nobuyoshi Shimizu, Tadashi Yamamoto, Kumao Toyoshima, Glenn T. Merlino, Young-hua Xu, Ira Pastan, Translocation chromosome 7 of A431 cells contains amplification and rearrangement of EGF receptor gene responsible for production of variant mRNA. Somatic Cell and Molecular Genetics. ,vol. 11, pp. 477- 484 ,(1985) , 10.1007/BF01534841
John M Chirgwin, Alan E Przybyla, Raymond J MacDonald, William J Rutter, None, Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. ,vol. 18, pp. 5294- 5299 ,(1979) , 10.1021/BI00591A005
Gamou Shinobu, Young S. Kim, Shimizu Nobuyoshi, Different responses to EGF in two human carcinoma cell lines, A431 and UCVA-1, possessing high numbers of EGF receptors Molecular and Cellular Endocrinology. ,vol. 37, pp. 205- 213 ,(1984) , 10.1016/0303-7207(84)90053-4
E.M. Southern, Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology. ,vol. 98, pp. 503- 517 ,(1975) , 10.1016/S0022-2836(75)80083-0
P. S. Thomas, Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 5201- 5205 ,(1980) , 10.1073/PNAS.77.9.5201
C V Rao, N Ramani, N Chegini, B K Stadig, F R Carman, P G Woost, G S Schultz, C L Cook, Topography of human placental receptors for epidermal growth factor. Journal of Biological Chemistry. ,vol. 260, pp. 1705- 1710 ,(1985) , 10.1016/S0021-9258(18)89651-X
Towia A. Libermann, Harris R. Nusbaum, Nissim Razon, Richard Kris, Irit Lax, Hermona Soreq, Nigel Whittle, Michael D. Waterfield, Axel Ullrich, Joseph Schlessinger, Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin Nature. ,vol. 313, pp. 144- 147 ,(1985) , 10.1038/313144A0
R. N. Fabricant, J. E. De Larco, G. J. Todaro, Nerve growth factor receptors on human melanoma cells in culture Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 565- 569 ,(1977) , 10.1073/PNAS.74.2.565
A. Ullrich, L. Coussens, J. S. Hayflick, T. J. Dull, A. Gray, A. W. Tam, J. Lee, Y. Yarden, T. A. Libermann, J. Schlessinger, J. Downward, E. L. V. Mayes, N. Whittle, M. D. Waterfield, P. H. Seeburg, Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells Nature. ,vol. 309, pp. 418- 425 ,(1984) , 10.1038/309418A0
J. Downward, Y. Yarden, E. Mayes, G. Scrace, N. Totty, P. Stockwell, A. Ullrich, J. Schlessinger, M. D. Waterfield, Close similarity of epidermal growth factor receptor and v- erb-B oncogene protein sequences Nature. ,vol. 307, pp. 521- 527 ,(1984) , 10.1038/307521A0