Identification of Key Pathways and Genes in the Orai2 Mediated Classical and Mesenchymal Subtype of Glioblastoma by Bioinformatic Analyses.

摘要: Background. Ca2+ release-activated channels (CRAC) are the main entry pathway regulating intracellular concentration in a variety of cancer types. Orai2 is pore-forming subunit CRAC central neurons. To explore role glioblastoma (GBM), we investigated key pathways and genes Orai2-mediated GBM by bioinformatic analyses. Methods. Via The Cancer Genome Atlas (TCGA), French, Sun, Gene Expression Omnibus (GEO) (GDS3885) datasets, collected 1231 cases with RNA-seq data analyzed functional annotation gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analysis. Univariate multivariate survival analyses were applied to 823 patients data. Results. We discovered that was markedly upregulated compared normal brain samples lower-grade gliomas (LGG). Survival analysis found higher expression independently associated worse prognosis classical mesenchymal subtypes GBM. Simultaneously, tumors than other significantly correlated classical- mesenchymal-related genes. GO KEGG revealed involved JNK pathway. Through screening transcriptomic data, strong association between apoptosis, stemness, an epithelial-mesenchymal transition- (EMT-) like phenotype. Conclusion. As prognostic factor, obviously activated promotes glioma cell self-renewal, EMT-like These findings indicate could be candidate therapeutic target, especially for