N-glycosylation Profiling of Colorectal Cancer Cell Lines Reveals Association of Fucosylation with Differentiation and Caudal Type Homebox 1 (CDX1)/Villin mRNA Expression

摘要: Various cancers such as colorectal cancer (CRC) are associated with alterations in protein glycosylation. CRC cell lines frequently used to study these (glyco)biological changes and their mechanisms. However, differences between regard glycosylation have hitherto been largely neglected. Here, we comprehensively characterized the N-glycan profiles of 25 different lines, derived from primary tumors metastatic sites, order investigate potential glycobiological tumor model systems reveal glycans line phenotypes. We applied an optimized, high-throughput membrane-based enzymatic glycan release for small sample amounts. Released were derivatized stabilize differentiate α2,3- α2,6-linked N-acetylneuraminic acids, followed by N-glycosylation analysis MALDI-TOF(/TOF)-MS. Our results showed pronounced patterns lines. differed tissue-derived high-mannose content but a large overlap complex type N-glycans, supporting use system. Importantly, could show that N-glycans did not only occur intracellular precursors also present at surface. The obtained features clearly correlated mRNA expression levels glycosyltransferases, demonstrating usefulness performing structural glycans. Finally, correlation markers phenotypes revealed association highly fucosylated CDX1 and/or villin both correlate differentiation. Together, our findings provide new insights into CRC-associated setting basis more in-depth experiments on function regulation.