Glycosylation characteristics of colorectal cancer

摘要: Glycans on proteins and lipids are known to alter with malignant transformation. The study of these may contribute the discovery biomarkers treatment targets as well understanding cancer biology. We here describe change glycosylation specifically defining colorectal view N-glycans, O-glycans, glycosphingolipid glycans in cells tissues patient sera. Glycan alterations observed colon include increased β1,6-branching correlating higher abundance (poly-)N-acetyllactosamine extensions N-glycans an increase (truncated) high-mannose type glycans, while bisected structures decrease. Colorectal cancer-associated O-glycan changes predominated by reduced expression core 3 4 whereas levels 1 (sialyl) T-antigen, Tn-antigen, a generally density O-glycans observed. Specific for lower abundances disialylated globo-type exception Gb3. In general, affecting all discussed glycan types sialylation, fucosylation Lewis-type antigens type-2 chain glycans. As consequence, interactions glycan-binding can be affected biological function cellular consequences altered regard tumorigenesis, metastasis, modulation immunity, resistance antitumor therapy will discussed. Finally, analytical approaches aiding field glycomics reviewed focus binding assays mass spectrometry.