Detection and characterization of interleukin-1 in human dental pulps

摘要: Pulp tissue was obtained from clinically intact third molars and premolars, teeth affected by caries periodontal disease. After incubation homogenization, supernatants were centrifuged, sampled assayed for lymphocyte activating factor-like activity using the thymidine-incorporated D10.G4.1 cell-line assay. Significantly higher levels of mitogenic found in pulp symptomatic carious teeth, those with attendant pericoronitis, than pulps asymptomatic periodontally premolars. As both interleukin-1 (IL-1) interleukin-2 (IL-2) may be involved proliferation cells, further tested IL-2 murine natural killer (NK) cells. Those that had elicited a proliferative response cells did not enhance NK proliferation, indicating factor responsible clonal replication IL-1. To confirm presence IL-1, immunohistochemistry monoclonal antibody to IL-1 performed on frozen paraffin-embedded sections each group. immunolocalized within The cell type producing characterized as an alpha-naphthyl-acetate-esterase-positive macrophage connective stroma This novel demonstration human dental indicates involvement this mediator inflammation