Impact of a Transposon Insertion in phzF2 on the Specialized Metabolite Production and Interkingdom Interactions of Pseudomonas aeruginosa
作者： V. V. Phelan , W. J. Moree , J. Aguilar , D. S. Cornett , A. Koumoutsi
DOI: 10.1128/JB.01258-13
关键词:
摘要: ABSTRACT In microbiology, gene disruption and subsequent experiments often center on phenotypic changes caused by one class of specialized metabolites (quorum sensors, virulence factors, or natural products), disregarding global downstream metabolic effects. With the recent development mass spectrometry-based methods technologies for microbial metabolomics investigations, it is now possible to visualize production diverse classes simultaneously. Using imaging spectrometry (IMS) applied analysis microbiology experiments, we can observe effects mutations, knockouts, insertions, complementation interactive metabolome. this study, a combination IMS liquid chromatography-tandem (LC-MS/MS) was used impact metabolite transposon insertion into Pseudomonas aeruginosa phenazine biosynthetic gene, phzF2 . The biosynthesis led broad in production, including loss pyoverdine production. This shift significantly alters outcome an interaction with Aspergillus fumigatus influencing triacetylfusarinine
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