Method for determining dna double-stranded breaks

作者: Emmy Rogakou , William M. Bonner

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摘要: The present invention provides a method and kit for determining double-stranded breaks in DNA. comprises contacting sample comprising H2A histone proteins with the isolated or purified antibody antigenically-reactive fragment thereof that specifically binds to C-terminal phosphorylated serine an protein, detecting binding of protein sample. detection indicates presence DNA break

参考文章(2)
Emmy P. Rogakou, Duane R. Pilch, Ann H. Orr, Vessela S. Ivanova, William M. Bonner, DNA Double-stranded Breaks Induce Histone H2AX Phosphorylation on Serine 139 Journal of Biological Chemistry. ,vol. 273, pp. 5858- 5868 ,(1998) , 10.1074/JBC.273.10.5858
Emmy P. Rogakou, Chye Boon, Christophe Redon, William M. Bonner, Megabase Chromatin Domains Involved in DNA Double-Strand Breaks in Vivo Journal of Cell Biology. ,vol. 146, pp. 905- 916 ,(1999) , 10.1083/JCB.146.5.905