Liquid-phase microextraction combined with capillary electrophoresis, a promising tool for the determination of chiral drugs in biological matrices.

摘要: Abstract A disposable device for liquid-phase microextraction (LPME) based on porous polypropylene hollow fibres has recently been introduced. In the present paper, LPME was combined with capillary electrophoresis (CE) and combination first time evaluated chiral determination of drugs in biological matrices. The antidepressant drug mianserin selected as model compound. enantiomers were extracted from 0.5 ml plasma added internal standard made alkaline 0.25 2 M NaOH. unionised analytes into di- n -hexyl ether impregnated pores fibre, an acidic solution inside fibre. This resulted a three-phase system where extracts aqueous, hence directly compatible CE system. Efficient sample clean-up seen extraction recovery 80% both enantiomers. Discrimination between not observed. limit quantitation ( S / N =10; 12.5 ng/ml enantiomers) detection =3; 4 below therapeutic range mianserin. method validated successfully applied to determine R - -mianserin samples seven patients treated mianserin, indicating that LPME–CE is promising analysis racemic low concentrations