作者: Kenneth E. Rubenstein , Edwin F. Ullman
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摘要: Novel biological assay method for determining the presence of a specific organic material by employing modified enzyme amplification. By receptors one or group materials (hereinafter referred to as "ligands") and binding an ligand counterfeit provide "enzyme-bound-ligand", extremely sensitive is provided assaying ligands. The receptor when bound enzyme-bound-ligand substantially inhibits enzymatic activity, providing different catalytic efficiencies combined with receptor. receptor, are in arbitrary order effect on activity determined. Various protocols may be used relating result amount present.