Adhesion of Tumor Cells to Hepatocytes: Different Mechanisms for Mammary Carcinoma Compared With Lymphosarcoma Cells

摘要: Mechanisms of adhesion between tumor cells and hepatocytes, which are likely to play a role in liver metastasis formation, were studied vitro. TA3 mammary carcinoma MB6A lymphosarcoma added rat hepatocytes that had been cultured for 24 hours. Adhesion was quantified by counting adherent seen sections pelleted, Epon-embedded culture fragments. TA3, but not cells, inhibited antibodies prepared from an antiserum raised against sinusoidal face-enriched plasma membranes. Detergent-solubilized components, affinity purified on immobilized inhibitory antibodies, neutralized inhibition, whereas subfraction separated this material with the use noninhibitory antiliver no neutralizing activity. calcium ionophore A23187 local anesthetic procaine. The calmodulin inhibitor trifluoperazine more strongly than cells. Finally, dependent external calcium, case could be replaced magnesium. These observations suggested two cell types involved distinct hepatocyte surface molecules required biochemical machinery.